Scientist III - Virology

Thermo Fisher Scientific Carlsbad, CA

About the Job

Job Title: Scientist III - Virology R&D
Requisition ID: 135859BR



When you’re part of the team at Thermo Fisher Scientific, you’ll do important work, like helping customers in finding cures for cancer, protecting the environment or making sure our food is safe. Your work will have real-world impact, and you’ll be supported in achieving your career goals.

Location/Division Specific Information
Carlsbad, CA | Biosciences Division

How will you make an impact?

Be part of the genome engineering revolution. This scientist will develop tools and techniques to support genome engineering. This scientist will be expected to work in a highly collaborative team environment, supporting some projects with some supervision of a senior scientist and leading some projects independently. Projects may include, but not be limited to, cloning, developing gene editing strategies, and developing assays to monitor editing.

What will you do?
  • Mammalian cell culture
  • DNA delivery and mammalian cell transfection
  • Generation of Lentivirus
  • Genomic editing in mammalian cell lines (TALN and CRISPR based editing, design and implementation)
  • Flow cytometric analysis
  • Plasmid subcloning, DNA preparations, sequencing, in vitro transcription of RNA, PCR
How will you get here?

Education
  • Master’s degree with 5+ years of experience in cell biology
  • PhD with 2+ years of experience in cell biology (preferred)
Experience
  • Proven experience in cell biology
    • Mammalian cell culture
    • Lipid-based cell transfection and electroporation
    • Cell engineering (CRISPR or TALEN)
    • Lentiviral vector construction and generation of lentivirus
    • Stable cell line generation
    • Cell-based assays, such as FACS, immunocytochemistry, ELISA, and Western Blotting
  • Experience in virology
    • Deliver packaging plasmids into cells
    • Generation of Lentivirus
    • Measurement of viral titers
    • PCR, qPCR assays
  • Experience in molecular biology
    • DNA cloning and sequencing
    • DNA, RNA preparation
    • PCR, qPCR assays
Knowledge, Skills, Abilities
  • Observe deviations from the expected outcomes and trouble-shoot
  • Succeed with a moderate level of supervision and guidance
  • Participate in a strong teamwork orientation with demonstrable influence/interpersonal skills
  • Maintain a detailed lab notebook and present your findings in a group setting

At Thermo Fisher Scientific, each one of our 70,000 extraordinary minds has a unique story to tell. Join us and contribute to our singular mission—enabling our customers to make the world healthier, cleaner and safer.

Apply today! http://jobs.thermofisher.com

Thermo Fisher Scientific is an EEO/Affirmative Action Employer and does not discriminate on the basis of race, color, religion, sex, sexual orientation, gender identity, national origin, protected veteran status, disability or any other legally protected status.